Real Results with Tru Niagen: What My TruDiagnostic Report Taught Me About NAD⁺ Optimization

Aug 6
3 min read

Updated: Aug 19

Real Results with Tru Niagen: What My TruDiagnostic Report Taught Me About NAD⁺ Optimization

Over the past several months, I’ve been supplementing with Tru Niagen®, a clinically proven NAD⁺ booster featuring nicotinamide riboside (NR) as its active ingredient. As someone passionate about longevity science and cellular health, I wanted to see how targeted supplementation could impact my biology.

I recently ran a deep-dive epigenetic test through TruDiagnostic’s TruHealth platform, which measures biological aging and other important health markers. The results were mostly great—my overall biological profile showed positive trends, and I felt strong confirmation that my wellness routine is moving in the right direction.

But one metric stood out and made me pause:

📊 Nicotinamide: High (595 ng/mL — 97th percentile)

This result placed me in the top 3% of the population in terms of circulating nicotinamide (NAM)—a form of vitamin B3 and a byproduct of NAD⁺ metabolism.

According to the TruHealth report:

"Nicotinamide is a form of vitamin B3, essential for NAD+ production and cellular energy. Low levels are associated with lower NAD+ production, and high levels are associated with inhibition of Sirtuins and NAD+ recycling pathways."

While I was pleased to see my NAD⁺ production was clearly upregulated, this result prompted a deeper investigation.

🧬 What Does High Nicotinamide Mean?

When you supplement with nicotinamide riboside (NR) — like in Tru Niagen® — your body converts it to NAD⁺, which is critical for cellular energy, DNA repair, inflammation regulation, and mitochondrial function. As NAD⁺ is used by key enzymes like sirtuins, PARPs, and CD38, it’s broken down into nicotinamide (NAM).

This is completely normal. But when supplementation is sustained or dosed too high, and the body's recycling pathways get overwhelmed, NAM can accumulate in the bloodstream.

While nicotinamide is protective at normal levels, excess NAM can be counterproductive:

It inhibits sirtuins, the enzymes that regulate aging, stress resistance, and mitochondrial health.
It can block NAMPT, a key enzyme in the NAD⁺ salvage pathway.
At high levels, it may contribute to inflammation and metabolic imbalance.

So yes — my high nicotinamide level was very likely a direct result of sustained Tru Niagen supplementation.

What Worked Well

Let’s not forget the upside: my NAD⁺ levels and biological markers were strong. That tells me Tru Niagen was effective in doing what it claims—boosting NAD⁺.

In fact, Tru Niagen® 300 mg has been clinically shown to increase NAD⁺ by up to 51% in just two weeks. Niagen Bioscience, the global leader behind this formula, has supported nearly 300 research studies across 200+ institutions in 30+ countries.

For me, it did exactly what it promised.

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What I’m Adjusting Now

Based on my results and research, it’s time to fine-tune my routine—not stop. I’m shifting from just boosting NAD⁺ to optimizing its metabolism and maintaining healthy sirtuin activity.

Here’s how I’m adjusting:

1. Lowering or Cycling My NR Dose

I’m reducing my daily dose of Tru Niagen from 300 mg to 150 mg
I’ll experiment with cycling: 5 days on, 2 days off, or 2 weeks on, 1 week off

2. Supporting Sirtuins

I’ve added Resveratrol (250 mg/day) and Quercetin (500 mg/day) to my routine. These compounds help activate sirtuins, even when NAM levels are elevated.

3. Clearing NAM Naturally

I’m supporting NAD⁺ clearance and recycling with:
- Alpha-Ketoglutarate (AKG) for mitochondrial detox
- Time-restricted eating (14–16 hours fasting)
- Regular sauna use and exercise, which naturally increase NAD⁺ turnover

4. Retesting in 2–3 Months

I’ll rerun the epigenetic test to track how these changes affect NAM levels and NAD⁺ balance

🧠 What I’ve Learned

This experience taught me that boosting NAD⁺ is just the beginning. Once you reach high cellular NAD⁺ levels, the next phase is about balance, recycling, and cellular intelligence.

The body knows how to regulate itself—our job is to support the pathways, not override them.

High-quality supplements like Tru Niagen are powerful tools. But even the best tools require personalized use, especially when we’re optimizing for longevity.

🌿 Final Takeaway

If you’re supplementing with NR or NMN, it’s worth testing your NAD⁺ metabolites to understand how your body is truly responding. Keep an eye out for signs of excess nicotinamide—and don’t hesitate to fine-tune your stack.